NYREN18 (NUB1) Mouse Monoclonal Antibody [Clone ID: OTI1F3]

CAT#: TA800184

NUB1 (NYREN18) mouse monoclonal antibody, clone OTI1F3 (formerly 1F3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NUB1 (RC208098, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NUB1. Positive lysates LY414176 (100ug) and LC414176 (20ug) can be purchased separately from OriGene.

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  HEK293T cells transfected with either RC208098 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-NUB1 antibody (TA800184), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI1F3
• Applications: FC, WB
• Recommended Dilution: WB 1:2000, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-326 of human NUB1 (NP_057202) produced in E.coli.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 68.9 kDa
• Gene Name: negative regulator of ubiquitin-like proteins 1
• Database Link:
  NP_057202
  Entrez Gene 53312 MouseEntrez Gene 296731 RatEntrez Gene 51667 Human
  UniProt ID Q9Y5A7
• Background: NUB1 interacts with and negatively regulates NEDD8 (MIM 603171), a ubiquitin-like protein that covalently conjugates to cullin (see MIM 603134) family members. [supplied by OMIM]
• Synonyms: BS4; NUB1L; NYREN18

Reference Data

• Protein Families: Druggable Genome