PSCA Rabbit Monoclonal Antibody [Clone ID: OTIR4B1]

CAT#: TA592756

PSCA Rabbit monoclonal antibody,clone OTIR4B1

Product Images

IHC staining of FFPE human prostatic hyperplasia tissue using anti-PSCA rabbit monoclonal antibody (TA592756) and Polink-2 HRP polymer detection kit (D22-110). Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 (B04C-100) at 120°C for 3 min. The brown stain indicates positive stain, blue is the counter stain.

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  IHC staining of FFPE human duodenum tissue within the normal limits using anti-PSCA rabbit monoclonal antibody (TA592756) and Polink-2 HRP polymer detection kit (D22-110). Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 (B04C-100) at 120°C for 3 min. The brown stain indicates positive stain, blue is the counter stain.

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  Western blot analysis of overexpressed lysates(15ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, lane 1) , human PSCA plasmid (RC209136, lane 2) using anti-PSCA antibody TA592756(1:3000).

Specifications

Product Data

• Clone Name: OTIR4B1
• Applications: IHC, WB
• Recommended Dilution: WB 1:3000, IHC 1:300
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human PSCA (NP_005663). The exact sequence is proprietary.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Shipped at -20°C or with ice packs, Upon delivery store at -20°C. Dilute in PBS(pH7.3) if necessary. Stable for 12 months from date of receipt. Avoid repeated freeze-thaws.
• Predicted Protein Size: 12.9 kDa
• Gene Name: prostate stem cell antigen
• Database Link:
  NP_005663
  Entrez Gene 8000 Human
  UniProt ID O43653
• Background: This gene encodes a glycosylphosphatidylinositol-anchored cell membrane glycoprotein. In addition to being highly expressed in the prostate it is also expressed in the bladder, placenta, colon, kidney, and stomach. This gene is up-regulated in a large proportion of prostate cancers and is also detected in cancers of the bladder and pancreas. This gene includes a polymorphism that results in an upstream start codon in some individuals; this polymorphism is thought to be associated with a risk for certain gastric and bladder cancers. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2010]
• Synonyms: PRO232