IDH2 Rabbit Monoclonal Antibody [Clone ID: OTIR5H7]

CAT#: TA592470

IDH2 R140Q Rabbit monoclonal antibody,clone OTIR5H7

Product Images

Figure A, Western blot analysis of lysates from HEK293T cells transfected with IDH2 R140Q mutant overexpression plasmid(Lane 2), IDH2 172S mutant overexpression plasmid(Lane 3) and IDH2 wild-type overexpression plasmid (RC201152, Lane 4) or empty vector plasmid (PS100001, Lane 1) using anti-IDH2 R140Q antibody, TA592470(1:500). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:1000).

Specifications

Product Data

• Clone Name: OTIR5H7
• Applications: WB
• Recommended Dilution: WB 1:500
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within DDK. The exact sequence is proprietary.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Shipped at -20°C or with ice packs, Upon delivery store at -20°C. Dilute in PBS(pH7.3) if necessary. Stable for 12 months from date of receipt. Avoid repeated freeze-thaws.
• Predicted Protein Size: 50.9 kDa
• Gene Name: isocitrate dehydrogenase (NADP(+)) 2
• Database Link:
  Entrez Gene 3418 Human
  UniProt ID P48735
• Background: Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]