Caspase-7 (CASP7) Rabbit Polyclonal Antibody

CAT#: TA590426

Rabbit Polyclonal Caspase 7 Antibody

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY CASP7 (Cat# RC221589, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CASP7 (Cat# TA590426). Positive lysates LY429870 (100ug) and LC429870 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CASP7 polyclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  ELISA: Caspase 7 Antibody - Affinity Purified

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CASP7 rabbit polyclonal antibody. (TA590426)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-CASP7 rabbit polyclonal antibody. (TA590426)

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Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: WB 1:5000~20000, IHC 1:150,ELISA 1:100-1:2000
• Reactivities: Human, Monkey, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: DNA immunization. This antibody is specific for the N Terminus Region of the target protein.
• Formulation: 20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0
• Concentration: 1.27153 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: caspase 7
• Database Link:
  NP_203124
  Entrez Gene 12369 MouseEntrez Gene 697633 MonkeyEntrez Gene 840 Human
  UniProt ID P55210
• Background: Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspases have the ability to cleave after aspartic acid residues. There are two classes of caspases involved in apoptosis; initiators (activation by receptor cluster) and effectors (activation by mitochondrial permeability transition). Proapoptotic signals autocatalytically activate initiator caspases, such as Caspase-8 and Caspase-9. Activated initiator caspases then process effector caspases, such as Caspase-3 and Caspase-7, which in turn cause cell collapse.
• Synonyms: CASP-7; CMH-1; ICE-LAP3; LICE2; MCH3
• Note: This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT

Reference Data

• Protein Families: Druggable Genome, Protease
• Protein Pathways: Alzheimer's disease, Apoptosis