UBE2S Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI1B5]

CAT#: TA505168AM

UBE2S mouse monoclonal antibody,clone 1B5, Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBE2S (RC224851, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UBE2S. Positive lysates LY402342 (100ug) and LC402342 (20ug) can be purchased separately from OriGene.

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  HEK293T cells transfected with either RC224851 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-UBE2S antibody (TA505168), and then analyzed by flow cytometry.

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  Flow cytometric Analysis of Hela cells, using anti-UBE2S antibody (TA505168), (Red), compared to a nonspecific negative control antibody, (Blue).

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  Flow cytometric Analysis of Jurkat cells, using anti-UBE2S antibody (TA505168), (Red), compared to a nonspecific negative control antibody, (Blue).

Specifications

Product Data

• Clone Name: OTI1B5
• Applications: FC, WB
• Recommended Dilution: WB 1:2000, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human UBE2S(NP_055316) produced in E.coli.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 23.7 kDa
• Gene Name: ubiquitin conjugating enzyme E2 S
• Database Link:
  NP_055316
  Entrez Gene 77891 MouseEntrez Gene 292588 RatEntrez Gene 27338 Human
  UniProt ID Q16763
• Background: This gene encodes a member of the ubiquitin-conjugating enzyme family. The encoded protein is able to form a thiol ester linkage with ubiquitin in a ubiquitin activating enzyme-dependent manner, a characteristic property of ubiquitin carrier proteins. [provided by RefSeq]. COMPLETENESS: complete on the 3' end.
• Synonyms: E2-EPF; E2EPF; EPF5

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Ubiquitin mediated proteolysis