FTCD Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI4F5]

CAT#: TA504951AM

FTCD mouse monoclonal antibody,clone 4F5, Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FTCD (RC208573, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FTCD. Positive lysates LY416500 (100ug) and LC416500 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-FTCD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504951)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-FTCD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504951)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-FTCD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504951)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-FTCD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504951)

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Specifications

Product Data

• Clone Name: OTI4F5
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human FTCD(NP_006648) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 58.7 kDa
• Gene Name: formimidoyltransferase cyclodeaminase
• Database Link:
  NP_006648
  Entrez Gene 10841 Human
  UniProt ID O95954
• Background: The protein encoded by this gene is a bifunctional enzyme that channels 1-carbon units from formiminoglutamate, a metabolite of the histidine degradation pathway, to the folate pool. Mutations in this gene are associated with glutamate formiminotransferase deficiency. Alternatively spliced transcript variants have been found for this gene.
• Synonyms: LCHC1

Reference Data

• Protein Pathways: Histidine metabolism, Metabolic pathways, One carbon pool by folate