METT10D (METTL16) Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI3E8]

CAT#: TA504665AM

METT10D mouse monoclonal antibody,clone 3E8, Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY METT10D (RC208648, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-METT10D. Positive lysates LY411358 (100ug) and LC411358 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-METT10D monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-METT10D mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA504665)

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  Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-METT10D mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA504665)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-METT10D mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA504665)

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Specifications

Product Data

• Clone Name: OTI3E8
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:500
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human METT10D(NP_076991) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 63.4 kDa
• Gene Name: methyltransferase 16, N6-methyladenosine
• Database Link:
  NP_076991
  Entrez Gene 67493 MouseEntrez Gene 79066 Human
  UniProt ID Q86W50
• Synonyms: METT10D