TULP3 Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI4E5]

CAT#: TA504149BM

TULP3 mouse monoclonal antibody, clone OTI4E5 (formerly 4E5), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY TULP3 (Cat# RC207595, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TULP3(Cat# TA504149). Positive lysates LY418767 (100ug) and LC418767 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-TULP3 monoclonal antibody.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-TULP3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504149)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TULP3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504149)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-TULP3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504149)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-TULP3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504149)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-TULP3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504149)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-TULP3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504149)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-TULP3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504149)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-TULP3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504149)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-TULP3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504149)

• Anti-TULP3 mouse monoclonal antibody (TA504149) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TULP3 (RC207595).

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Specifications

Product Data

• Clone Name: OTI4E5
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, IF 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human TULP3(NP_003315) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 49.5 kDa
• Gene Name: TUB like protein 3
• Database Link:
  NP_003315
  Entrez Gene 7289 Human
  UniProt ID O75386
• Background: This gene encodes a member of the tubby gene family of bipartite transcription factors. Members of this family have been identified in plants, vertebrates, and invertebrates, and they share a conserved N-terminal transcription activation region and a conserved C-terminal DNA and phosphatidylinositol-phosphate binding region. The encoded protein binds to phosphoinositides in the plasma membrane via its C-terminal region and probably functions as a membrane-bound transcription regulator that translocates to the nucleus in response to phosphoinositide hydrolysis, for instance, induced by G-protein-coupled-receptor signaling. It plays an important role in neuronal development and function. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]
• Synonyms: TUBL3

Reference Data

• Protein Families: Druggable Genome, Transcription Factors