RNF113B Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI1B10]

CAT#: TA504133BM

RNF113B mouse monoclonal antibody, clone OTI1B10 (formerly 1B10), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RNF113B (RC205407, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RNF113B. Positive lysates LY405825 (100ug) and LC405825 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-RNF113B monoclonal antibody.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-RNF113B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504133)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-RNF113B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504133)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-RNF113B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504133)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-RNF113B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504133)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-RNF113B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504133)

• Anti-RNF113B mouse monoclonal antibody (TA504133) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RNF113B (RC205407).

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Specifications

Product Data

• Clone Name: OTI1B10
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, IF 1:100
• Reactivities: Human, Dog, Rat, Monkey, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recomnbinant protein of human RNF113B(NP_849192) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 36.1 kDa
• Gene Name: ring finger protein 113B
• Database Link:
  NP_849192
  Entrez Gene 697578 MonkeyEntrez Gene 140432 Human
  UniProt ID Q8IZP6
• Synonyms: bA10G5.1; RNF161; ZNF183L1

Reference Data

• Protein Families: Druggable Genome