IFI35 Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI1C9]

CAT#: TA503386BM

IFI35 mouse monoclonal antibody, clone OTI1C9 (formerly 1C9), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY IFI35 (Cat# RC200929, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IFI35(Cat# TA503386). Positive lysates LY401696 (100ug) and LC401696 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 8 different cell lines by using anti-IFI35 monoclonal antibody (1:200).

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-IFI35 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503386)

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  Anti-IFI35 mouse monoclonal antibody (TA503386) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY IFI35 (RC200929).

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  HEK293T cells transfected with either RC200929 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-IFI35 antibody (TA503386), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI1C9
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human IFI35(NP_005524)produced in HEk293T cell
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 31.6 kDa
• Gene Name: interferon induced protein 35
• Database Link:
  NP_005524
  Entrez Gene 3430 Human
  UniProt ID P80217
• Synonyms: IFP35