PDE1B Mouse Monoclonal Antibody [Clone ID: OTI6E4]

CAT#: TA503319

PDE1B mouse monoclonal antibody, clone OTI6E4 (formerly 6E4)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY PDE1B (Cat# RC206588, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDE1B(Cat# TA503319). Positive lysates LY400337 (100ug) and LC400337 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PDE1B monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PDE1B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503319)

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  Anti-PDE1B mouse monoclonal antibody (TA503319) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PDE1B (RC206588).

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  HEK293T cells transfected with either RC206588 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PDE1B antibody (TA503319), and then analyzed by flow cytometry.

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  Flow cytometric Analysis of Jurkat cells, using anti-PDE1B antibody (TA503319), (Red), compared to a nonspecific negative control antibody, (Blue).

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Specifications

Product Data

• Clone Name: OTI6E4
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PDE1B(NP_000915) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 61.2 kDa
• Gene Name: phosphodiesterase 1B
• Database Link:
  NP_000915
  Entrez Gene 18574 MouseEntrez Gene 29691 RatEntrez Gene 5153 Human
  UniProt ID Q01064
• Background: Cyclic nucleotide phosphodiesterases (PDEs) catalyze hydrolysis of the cyclic nucleotides cAMP and cGMP to the corresponding nucleoside 5-prime-monophosphates. Mammalian PDEs have been classified into several families based on their biochemical properties. Members of the PDE1 family, such as PDE1B, are calmodulin (see MIM 114180)-dependent PDEs (CaM-PDEs) that are stimulated by a calcium-calmodulin complex (Repaske et al., 1992 [PubMed 1326532]). [supplied by OMIM]
• Synonyms: PDE1B1; PDES1B

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Calcium signaling pathway, Progesterone-mediated oocyte maturation, Purine metabolism