SIRT5 Mouse Monoclonal Antibody [Clone ID: OTI2F1]

CAT#: TA503232S

SIRT5 mouse monoclonal antibody, clone OTI2F1 (formerly 2F1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY SIRT5 (Cat# RC200189, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SIRT5(Cat# TA503232). Positive lysates LY415885 (100ug) and LC415885 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SIRT5 monoclonal antibody.

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and SIRT5-Knockout HeLa cells (KO, Cat# LC830483) were separated by SDS-PAGE and immunoblotted with anti-SIRT5 monoclonal antibody TA503232 (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-SIRT5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503232)

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  Anti-SIRT5 mouse monoclonal antibody (TA503232) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SIRT5 (RC200189).

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  HEK293T cells transfected with either RC200189 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SIRT5 antibody (TA503232), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI2F1
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Rat, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human SIRT5(NP_036373) produced in HEK293 cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.24 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 33.7 kDa
• Gene Name: sirtuin 5
• Database Link:
  NP_036373
  Entrez Gene 68346 MouseEntrez Gene 306840 RatEntrez Gene 23408 Human
  UniProt ID Q9NXA8
• Background: This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class III of the sirtuin family. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq]
• Synonyms: SIR2L5

Reference Data

• Protein Families: Druggable Genome, Transcription Factors