eRF1 (ETF1) Mouse Monoclonal Antibody [Clone ID: OTI2E3]

CAT#: TA502891

ETF1 (eRF1) mouse monoclonal antibody, clone OTI2E3 (formerly 2E3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY ETF1 (Cat# RC220387, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ETF1(Cat# TA502891). Positive lysates LY401492 (100ug) and LC401492 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ETF1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).

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  Flow cytometric Analysis of Hela cells, using anti-ETF1 antibody (TA502891), (Red), compared to a nonspecific negative control antibody, (Blue).

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  Flow cytometric Analysis of Jurkat cells, using anti-ETF1 antibody (TA502891), (Red), compared to a nonspecific negative control antibody, (Blue).

Specifications

Product Data

• Clone Name: OTI2E3
• Applications: FC, WB
• Recommended Dilution: WB 1:500, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ETF1 (NP_004721) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.97 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 48.9 kDa
• Gene Name: eukaryotic translation termination factor 1
• Database Link:
  NP_004721
  Entrez Gene 307503 RatEntrez Gene 2107 Human
  UniProt ID P62495
• Background: This gene encodes a class-1 polypeptide chain release factor. The encoded protein plays an essential role in directing termination of mRNA translation from the termination codons UAA, UAG and UGA. This protein is a component of the SURF complex which promotes degradation of prematurely terminated mRNAs via the mechanism of nonsense-mediated mRNA decay (NMD). Alternate splicing results in coding and non-coding transcript variants. Pseudogenes of this gene are found on chromosomes 5, 7 and X. [provided by RefSeq, Jan 2012]
• Synonyms: D5S1995; ERF; ERF1; RF1; SUP45L1; TB3-1