LMCD1 Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI2F12]

CAT#: TA502789AM

LMCD1 mouse monoclonal antibody, clone OTI2F12 (formerly 2F12), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LMCD1 (RC200062, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LMCD1. Positive lysates LY402349 (100ug) and LC402349 (20ug) can be purchased separately from OriGene.

• 

  HEK293T cells transfected with either RC200062 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LMCD1 antibody (TA502789), and then analyzed by flow cytometry.

• 

  Flow cytometric Analysis of Hela cells, using anti-LMCD1 antibody (TA502789), (Red), compared to a nonspecific negative control antibody, (Blue).

Specifications

Product Data

• Clone Name: OTI2F12
• Applications: FC, WB
• Recommended Dilution: WB 1:500, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human LMCD1 (NP_055398) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 40.7 kDa
• Gene Name: LIM and cysteine rich domains 1
• Database Link:
  NP_055398
  Entrez Gene 29995 Human
  UniProt ID Q9NZU5
• Background: The protein encoded by this gene contains a cysteine-rich domain in the N-terminal region and 2 LIM domains in the C-terminal region. It also has several potential phosphorylation and N-myristoylation sites and a single potential N-glycosylation site. The presence of LIM domains implies involvement in protein-protein interactions. Expression of this gene has been detected in most tissues, with highest expression in skeletal muscle. Transcript variants utilizing alternative polyA signals have been observed. [provided by RefSeq]. COMPLETENESS: complete on the 3' end.
• Synonyms: dyxin; LIM and cysteine-rich domains 1