Glutathione Synthetase (GSS) Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI1B8]

CAT#: TA502094BM

GSS (Glutathione Synthetase) mouse monoclonal antibody, clone OTI1B8 (formerly 1B8), HRP conjugated

Product Images

HEK293T cells transfected with either RC203174 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-GSS antibody (TA502094), and then analyzed by flow cytometry.

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY GSS (Cat# RC203174, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GSS(Cat# TA502094). Positive lysates LY424876 (100ug) and LC424876 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-GSS mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502094)

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-GSS mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502094)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-GSS mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502094)

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Specifications

Product Data

• Clone Name: OTI1B8
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human GSS (NP_000169) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 52.2 kDa
• Gene Name: glutathione synthetase
• Database Link:
  NP_000169
  Entrez Gene 14854 MouseEntrez Gene 25458 RatEntrez Gene 2937 Human
  UniProt ID P48637
• Background: Glutathione is important for a variety of biological functions, including protection of cells from oxidative damage by free radicals, detoxification of xenobiotics, and membrane transport. The protein encoded by this gene functions as a homodimer to catalyze the second step of glutathione biosynthesis, which is the ATP-dependent conversion of gamma-L-glutamyl-L-cysteine to glutathione. Defects in this gene are a cause of glutathione synthetase deficiency. [provided by RefSeq]
• Synonyms: GSHS; HEL-S-64p; HEL-S-88n

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Glutathione metabolism, Metabolic pathways