GRHPR Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI4B6]

CAT#: TA501890BM

GRHPR mouse monoclonal antibody, clone OTI4B6 (formerly 4B6), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GRHPR (RC200963, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GRHPR. Positive lysates LY415912 (100ug) and LC415912 (20ug) can be purchased separately from OriGene.

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT, Cat# LC810293T) and GRHPR-Knockout 293T cells (KO, Cat# LC841943 ) were separated by SDS-PAGE and immunoblotted with anti-GRHPR monoclonal antibody TA501890 (1:200`). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-GRHPR mouse monoclonal antibody at 1:150 (TA501890)

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  HEK293T cells transfected with either RC200963 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-GRHPR antibody (TA501890), and then analyzed by flow cytometry.

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  Flow cytometric Analysis of Hela cells, using anti-GRHPR antibody (TA501890), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

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Specifications

Product Data

• Clone Name: OTI4B6
• Applications: FC, IHC, WB
• Recommended Dilution: WB: 1:2000, FLOW: 1:100, IHC: 1:50-1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human GRHPR (NP_036335) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 35.5 kDa
• Gene Name: glyoxylate and hydroxypyruvate reductase
• Database Link:
  NP_036335
  Entrez Gene 680021 RatEntrez Gene 9380 Human
  UniProt ID Q9UBQ7
• Background: This gene encodes an enzyme with hydroxypyruvate reductase, glyoxylate reductase, and D-glycerate dehydrogenase enzymatic activities. The enzyme has widespread tissue expression and has a role in metabolism. Type II hyperoxaluria is caused by mutations in this gene. [provided by RefSeq]
• Synonyms: GLXR; GLYD; PH2

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Glyoxylate and dicarboxylate metabolism, Metabolic pathways, Pyruvate metabolism