GGPS1 Mouse Monoclonal Antibody [Clone ID: OTI3F9]

CAT#: TA501731

GGPS1 mouse monoclonal antibody, clone OTI3F9 (formerly 3F9)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY GGPS1 (Cat# RC202699, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GGPS1(Cat# TA501731). Positive lysates LY401517 (100ug) and LC401517 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-GGPS1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).

• 

  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-GGPS1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501731)

• 

  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-GGPS1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501731)

Specifications

Product Data

• Clone Name: OTI3F9
• Applications: IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human GGPS1 (NP_004828) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.81 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 34.7 kDa
• Gene Name: geranylgeranyl diphosphate synthase 1
• Database Link:
  NP_004828
  Entrez Gene 14593 MouseEntrez Gene 291211 RatEntrez Gene 9453 Human
  UniProt ID O95749
• Background: This gene is a member of the prenyltransferase family and encodes a protein with geranylgeranyl diphosphate (GGPP) synthase activity. The enzyme catalyzes the synthesis of GGPP from farnesyl diphosphate and isopentenyl diphosphate. GGPP is an important molecule responsible for the C20-prenylation of proteins and for the regulation of a nuclear hormone receptor. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]
• Synonyms: GGPPS; GGPPS1

Reference Data

• Protein Pathways: Metabolic pathways, Terpenoid backbone biosynthesis