BCL10 Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI4A8]

CAT#: TA501237BM

BCL10 mouse monoclonal antibody, clone 4A8, HRP conjugated

Product Images

Anti-BCL10 mouse monoclonal antibody (TA501237) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BCL10 (RC208752 ).

• 

  HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BCL10 (RC208752, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BCL10. Positive lysates LY418351 (100ug) and LC418351 (20ug) can be purchased separately from OriGene.

• 

  Flow cytometric Analysis of Jurkat cells, using anti-BCL10 antibody (TA501237), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

• 

  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-BCL10 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501237, Dilution 1:50)

• 

  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-BCL10 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501237, Dilution 1:50)

• 

  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-BCL10 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501237, Dilution 1:50)

Show all 6 images

Specifications

Product Data

• Clone Name: OTI4A8
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:50, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human BCL10 (NP_003912) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 26.1 kDa
• Gene Name: BCL10 immune signaling adaptor
• Database Link:
  NP_003912
  Entrez Gene 12042 MouseEntrez Gene 83477 RatEntrez Gene 8915 Human
  UniProt ID O95999
• Background: This gene was identified by its translocation in a case of mucosa-associated lymphoid tissue (MALT) lymphoma. The protein encoded by this gene contains a caspase recruitment domain (CARD), and has been shown to induce apoptosis and to activate NF-kappaB. This protein is reported to interact with other CARD domain containing proteins including CARD9, 10, 11 and 14, which are thought to function as upstream regulators in NF-kappaB signaling. This protein is found to form a complex with MALT1, a protein encoded by another gene known to be translocated in MALT lymphoma. MALT1 and this protein are thought to synergize in the activation of NF-kappaB, and the deregulation of either of them may contribute to the same pathogenetic process that leads to the malignancy.
• Synonyms: c-E10; CARMEN; CIPER; CLAP; IMD37; mE10

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: B cell receptor signaling pathway, T cell receptor signaling pathway