Grp75 (HSPA9) Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI4A1]

CAT#: TA500548AM

Anti-HSPA9 mouse monoclonal antibody, clone OTI4A1 (formerly 4A1), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSPA9 (RC201397, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSPA9. Positive lysates LY401334 (100ug) and LC401334 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HSPA9 monoclonal antibody.

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  Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-HSPA9 monoclonal antibody at 1:500 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).

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  Anti-HSPA9 mouse monoclonal antibody (TA500548) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HSPA9 (RC201397).

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  Immunofluorescent staining of HT29 cells using anti-HSPA9 mouse monoclonal antibody (TA500548).

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Specifications

Product Data

• Clone Name: OTI4A1
• Applications: IF, WB
• Recommended Dilution: WB 1:2000, IF 1:100
• Reactivities: Human, Dog, Rat, Monkey, Mouse
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full-length protein expressed in 293T cell transfected with human HSPA9 expression vector
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 73.7 kDa
• Gene Name: heat shock protein family A (Hsp70) member 9
• Database Link:
  NP_004125
  Entrez Gene 15526 MouseEntrez Gene 291671 RatEntrez Gene 474697 DogEntrez Gene 714506 MonkeyEntrez Gene 3313 Human
  UniProt ID P38646
• Background: The product encoded by this gene belongs to the heat shock protein 70 family which contains both heat-inducible and constitutively expressed members. The latter are called heat-shock cognate proteins. This gene encodes a heat-shock cognate protein. This protein plays a role in the control of cell proliferation. It may also act as a chaperone.
• Synonyms: CSA; GRP-75; GRP75; HEL-S-124m; HSPA9B; MOT; MOT2; MTHSP75; PBP74

Reference Data

• Protein Families: Stem cell - Pluripotency
• Protein Pathways: RNA degradation