TXN Rabbit Polyclonal Antibody

CAT#: TA382955

Thioredoxin 1 (Trx1/TXN) Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using Thioredoxin 1 (Trx1/Thioredoxin 1 (Trx1/TXN)) antibody (TA382955) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 90s.

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  Immunofluorescence analysis of HeLa cells using Thioredoxin 1 (Trx1/Thioredoxin 1 (Trx1/TXN)) Rabbit pAb (TA382955) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Specifications

Product Data

• Applications: ICC/IF, IHC, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IHC,1:50 - 1:200
  IF,1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 20 to the C-terminus of human Thioredoxin 1 (Trx1/Thioredoxin 1 (Trx1/TXN)) (NP_003320.2).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 9kDa/11kDa
• Gene Name: thioredoxin
• Database Link:
  Entrez Gene 7295 Human
  UniProt ID P10599
• Background: The protein encoded by this gene acts as a homodimer and is involved in many redox reactions. The encoded protein is active in the reversible S-nitrosylation of cysteines in certain proteins, which is part of the response to intracellular nitric oxide. This protein is found in the cytoplasm. Two transcript variants encoding different isoforms have been found for this gene.
• Synonyms: ADF; DKFZp686B1993; MGC61975; SASP; thioredoxin; TRDX; TRX; TRX1