CIKS (TRAF3IP2) Rabbit Polyclonal Antibody

CAT#: TA382776

TRAF3IP2 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of Rat lung, using TRAF3IP2 antibody (TA382776) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 90s.

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  Western blot analysis of extracts of various cell lines, using TRAF3IP2 antibody (TA382776) at 1:500 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 180s.

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  Immunofluorescence analysis of NIH/3T3 cells using TRAF3IP2 Rabbit pAb (TA382776) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of PC-12 cells using TRAF3IP2 Rabbit pAb (TA382776) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of U2OS cells using TRAF3IP2 Rabbit pAb (TA382776) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

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Specifications

Product Data

• Applications: ICC/IF, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IF,1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human TRAF3IP2 (NP_679211.2).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 12kDa/18kDa/63kDa/64kDa
• Gene Name: TRAF3 interacting protein 2
• Database Link:
  Entrez Gene 10758 Human
  UniProt ID O43734
• Background: This gene encodes a protein involved in regulating responses to cytokines by members of the Rel/NF-kappaB transcription factor family. These factors play a central role in innate immunity in response to pathogens, inflammatory signals and stress. This gene product interacts with TRAF proteins (tumor necrosis factor receptor-associated factors) and either I-kappaB kinase or MAP kinase to activate either NF-kappaB or Jun kinase. Several alternative transcripts encoding different isoforms have been identified. Another transcript, which does not encode a protein and is transcribed in the opposite orientation, has been identified. Overexpression of this transcript has been shown to reduce expression of at least one of the protein encoding transcripts, suggesting it has a regulatory role in the expression of this gene.
• Synonyms: ACT1; C6orf2; C6orf4; C6orf5; C6orf6; CIKS; DKFZP586G0522; MGC3581