ACK1 (TNK2) Rabbit Polyclonal Antibody

CAT#: TA382672

Phospho-TNK2-Y284 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of HepG2 cells, using Phospho-TNK2-Y284 antibody (TA382672) at 1:1000 dilution. HepG2 cells were treated by EGF (100ng/mL) for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB,1:500 - 1:2000
• Reactivities: Human
• Modifications: Phospho Y284
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic phosphorylated peptide around Y284 of human TNK2 (NP_005772.3).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 60kDa/114kDa/119kDa
• Gene Name: tyrosine kinase non receptor 2
• Database Link:
  Entrez Gene 10188 Human
  UniProt ID Q07912
• Background: This gene encodes a tyrosine kinase that binds Cdc42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42Hs. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain. The protein may be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42Hs and which is directly linked to a tyrosine phosphorylation signal transduction pathway. Several alternatively spliced transcript variants have been identified from this gene, but the full-length nature of only two transcript variants has been determined.
• Synonyms: ACK; ACK-1; ACK1; FLJ44758; FLJ45547; p21cdc42Hs