STAT1 Rabbit Polyclonal Antibody

CAT#: TA382086

STAT1 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of HeLa cells, using STAT1 antibody (TA382086) at 1:500 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 30s.

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  Western blot analysis of extracts of various cell lines, using STAT1 antibody (TA382086) at 1:500 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit .|Exposure time: 180.

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  Immunofluorescence analysis of L929 cells using STAT1 Rabbit pAb (TA382086) at dilution of 1:100. Blue: DAPI for nuclear staining.

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  Immunoprecipitation analysis of 200ug extracts of U-87MG cells using 3ug STAT1 antibody (TA382086). Western blot was performed from the immunoprecipitate using STAT1 antibody (TA382086) at a dilition of 1:1000.

Specifications

Product Data

• Applications: ICC/IF, IHC, IP, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IHC,1:50 - 1:200
  IF,1:50 - 1:200
  IP,1:20 - 1:50
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 650-750 of human STAT1 (NP_009330.1).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 83kDa/87kDa
• Gene Name: signal transducer and activator of transcription 1
• Database Link:
  Entrez Gene 6772 Human
  UniProt ID P42224
• Background: The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. Two alternatively spliced transcript variants encoding distinct isoforms have been described.
• Synonyms: DKFZp686B04100; ISGF-3; OTTHUMP00000205845; STAT91