SSBP1 Rabbit Polyclonal Antibody

CAT#: TA382030

SSBP1 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using SSBP1 antibody (TA382030) at 1:500 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 30s.

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  Western blot analysis of extracts of Mouse liver, using SSBP1 antibody (TA382030) at 1:500 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit .|Exposure time: 90s.

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  Immunofluorescence analysis of U2OS cells using SSBP1 antibody (TA382030). Blue: DAPI for nuclear staining.

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  Confocal immunofluorescence analysis of U2OS cells using SSBP1 Polyclonal Antibody (TA382030) at dilution of 1:100. Blue: DAPI for nuclear staining.

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  Immunoprecipitation analysis of 200ug extracts of MCF7 cells using 1ug SSBP1 antibody (TA382030). Western blot was performed from the immunoprecipitate using SSBP1 antibody (TA382030) at a dilition of 1:1000.

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Specifications

Product Data

• Applications: ICC/IF, IP, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IF,1:50 - 1:100
  IP,1:50 - 1:200
• Reactivities: Human, Mouse
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-148 of human SSBP1 (NP_001243442.1).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 17kDa
• Gene Name: single stranded DNA binding protein 1
• Database Link:
  Entrez Gene 6742 Human
  UniProt ID Q04837
• Background: SSBP1 is a housekeeping gene involved in mitochondrial biogenesis (Tiranti et al., 1995 [PubMed 7789991]). It is also a subunit of a single-stranded DNA (ssDNA)-binding complex involved in the maintenance of genome stability (Huang et al., 2009) [PubMed 19683501].[supplied by OMIM, Feb 2010]
• Synonyms: Mt-SSB; MtSSB; SOSS-B1; SSBP