FYN Rabbit Polyclonal Antibody

CAT#: TA381988

Phospho-SRC Family-Y416 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of NIH/3T3 cells, using Phospho-SRC Family-Y416 pAb (TA381988) at 1:1000 dilution or SRC antibody ( A18240).NIH/3T3 cells were treated by PDGF (100 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit .|Exposure time: 60s.

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  Western blot analysis of extracts of NIH/3T3-Src cells, using Phospho-SRC Family-Y416 antibody (TA381988).|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB,1:500 - 1:2000
• Reactivities: Human, Mouse, Rat
• Modifications: Phospho Y416
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A phospho specific peptide corresponding to residues surrounding Y416 of human SRC Family
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 59kDa/60kDa
• Gene Name: FYN proto-oncogene, Src family tyrosine kinase
• Database Link:
  Entrez Gene 2534 Human
  UniProt ID P06241
• Background: This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.
• Synonyms: MGC45350; OTTHUMP00000017917; p59-Fyn; SLK; SYN