SRC Rabbit Polyclonal Antibody

CAT#: TA381984

Src Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of SH-SY5Y cells, using Src Rabbit pAb (TA381984) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 3s.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB,1:500 - 1:1000
  IP,1:50 - 1:200
• Reactivities: Human
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 410-536 of human Src (NP_005408.1).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 59kDa/60kDa
• Gene Name: SRC proto-oncogene, non-receptor tyrosine kinase
• Database Link:
  Entrez Gene 6714 Human
  UniProt ID P12931
• Background: This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.
• Synonyms: ASV; c-src; OTTHUMP00000174476; OTTHUMP00000174477; p60-Src; pp60c-src; SRC1