SIRT1 Rabbit Polyclonal Antibody

CAT#: TA381506

SIRT1 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts from normal (control) and SIRT1 knockout (KO) 293T cells, using SIRT1 antibody (TA381506) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 1s.

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  Western blot analysis of extracts of various cell lines, using SIRT1 antibody (TA381506) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 30s.

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  Immunofluorescence analysis of C6 cells using SIRT1 antibody (TA381506) at dilution of 1:100. Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of NIH-3T3 cells using SIRT1 antibody (TA381506) at dilution of 1:100. Blue: DAPI for nuclear staining.

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  Immunoprecipitation analysis of 300ug extracts of 293T cells using 3ug SIRT1 antibody (TA381506). Western blot was performed from the immunoprecipitate using SIRT1 antibody (TA381506) at a dilition of 1:1000.

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  Chromatin immunoprecipitation analysis of extracts of MCF7 cells, using SIRT1 antibody (TA381506) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

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Specifications

Product Data

• Applications: ChIP, ICC/IF, IP, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IHC,1:50 - 1:200
  IF,1:50 - 1:200
  IP,1:50 - 1:200
  ChIP,1:50 - 1:200
• Reactivities: Human, Mouse
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic peptide of human SIRT1
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 61kDa/81kDa
• Gene Name: sirtuin 1
• Database Link:
  Entrez Gene 23411 Human
  UniProt ID Q96EB6
• Background: This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants.
• Synonyms: hSIR2; hSIRT1; SIR2alpha; SIR2L1