PYGL Rabbit Polyclonal Antibody

CAT#: TA380591

PYGL Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using PYGL antibody (TA380591) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 1s.

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  Immunofluorescence analysis of HeLa cells using PYGL Rabbit pAb (TA380591) at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of HepG2 cells using PYGL Rabbit pAb (TA380591) at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.

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  Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug PYGL antibody (TA380591). Western blot was performed from the immunoprecipitate using PYGL antibody (TA380591) at a dilition of 1:1000.

Specifications

Product Data

• Applications: ICC/IF, IHC, IP, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IHC,1:50 - 1:200
  IF,1:50 - 1:200
  IP,1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human PYGL (NP_002854.3).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 93kDa/97kDa
• Gene Name: phosphorylase, glycogen, liver
• Database Link:
  Entrez Gene 5836 Human
  UniProt ID P06737
• Background: This gene encodes a homodimeric protein that catalyses the cleavage of alpha-1,4-glucosidic bonds to release glucose-1-phosphate from liver glycogen stores. This protein switches from inactive phosphorylase B to active phosphorylase A by phosphorylation of serine residue 15. Activity of this enzyme is further regulated by multiple allosteric effectors and hormonal controls. Humans have three glycogen phosphorylase genes that encode distinct isozymes that are primarily expressed in liver, brain and muscle, respectively. The liver isozyme serves the glycemic demands of the body in general while the brain and muscle isozymes supply just those tissues. In glycogen storage disease type VI, also known as Hers disease, mutations in liver glycogen phosphorylase inhibit the conversion of glycogen to glucose and results in moderate hypoglycemia, mild ketosis, growth retardation and hepatomegaly. Alternative splicing results in multiple transcript variants encoding different isoforms.
• Synonyms: GSD6