PP2A-alpha (PPP2CA) Rabbit Polyclonal Antibody

CAT#: TA380255

Phospho-PPP2CA/PPP2CB-Y307 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cells, using Phospho-PPP2CA/PPP2CB-Y307 antibody (TA380255) at 1:1000 dilution.HeLa cells were treated by Calyculin A (100nM) for 30 minutes after serum-starvation overnight.NIH/3T3 cells were treated by Calyculin A (100nM) for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit .|Exposure time: 1s.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB,1:500 - 1:2000
• Reactivities: Human, Mouse
• Modifications: Phospho Y307
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A phospho specific peptide corresponding to residues surrounding Y307 of human PPP2CA/PPP2CB
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Gene Name: protein phosphatase 2 catalytic subunit alpha
• Database Link:
  Entrez Gene 5515 Human
  UniProt ID P67775
• Background: This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit. [provided by RefSeq, Jul 2008]
• Synonyms: PP2A-alpha; PP2Ac; PP2CA; PP2Calpha; RP-C