PPIG Rabbit Polyclonal Antibody

CAT#: TA380214

Phospho-PPIG-S376 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using Phospho-PPIG-S376 antibody (TA380214) at 1:1000 dilution. 293T cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37℃ for 1 hour. C2C12 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37℃ for 1 hour. PC-12 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37℃ for 1 hour.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit .|Exposure time: 90s.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB,1:500 - 1:2000
• Reactivities: Human, Mouse, Rat
• Modifications: Phospho S376
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic phosphorylated peptide around S376 of human PPIG (NP_004783.2).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 40kDa/88kDa
• Gene Name: peptidylprolyl isomerase G
• Database Link:
  Entrez Gene 9360 Human
  UniProt ID Q13427
• Background: PPIase that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and may therefore assist protein folding (PubMed:20676357). May be implicated in the folding, transport, and assembly of proteins. May play an important role in the regulation of pre-mRNA splicing.
• Synonyms: CARS-cyclophilin; CARS-Cyp; CASP10; CYP; MGC133241; SR-cyclophilin; SR-cyp; SRcyp