PI 3 Kinase p85 alpha (PIK3R1) Rabbit Polyclonal Antibody

CAT#: TA379938

PI3 Kinase p85 alpha Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using PI3 Kinase p85 alpha antibody (TA379938) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 10s.

• 

  Immunofluorescence analysis of PC-12 cells using PI3 Kinase p85 alpha antibody (TA379938) at dilution of 1:100. Blue: DAPI for nuclear staining.

• 

  Immunofluorescence analysis of NIH/3T3 cells using PI3 Kinase p85 alpha antibody (TA379938) at dilution of 1:100. Blue: DAPI for nuclear staining.

Specifications

Product Data

• Applications: ICC/IF, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IF,1:50 - 1:200
• Reactivities: Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 240-380 of human PI3 Kinase p85 alpha (NP_852556.2).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 42kDa/49kDa/53kDa/83kDa/84kDa
• Gene Name: phosphoinositide-3-kinase regulatory subunit 1
• Database Link:
  UniProt ID P27986
• Background: Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms.
• Synonyms: GRB1; p85; p85-ALPHA; PI3K