PGK2 Rabbit Polyclonal Antibody

CAT#: TA379846

PGK2 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using PGK2 antibody (TA379846) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 5s.

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  Immunofluorescence analysis of C6 cells using PGK2 Polyclonal Antibody (TA379846) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of L929 cells using PGK2 Polyclonal Antibody (TA379846) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Specifications

Product Data

• Applications: ICC/IF, IHC, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IHC,1:50 - 1:100
  IF,1:50 - 1:100
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 242-360 of human PGK2 (NP_620061.2).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 44kDa
• Gene Name: phosphoglycerate kinase 2
• Database Link:
  Entrez Gene 5232 Human
  UniProt ID P07205
• Background: This gene is intronless, arose via retrotransposition of the phosphoglycerate kinase 1 gene, and is expressed specifically in the testis. Initially assumed to be a pseudogene, the encoded protein is actually a functional phosphoglycerate kinase that catalyzes the reversible conversion of 1,3-bisphosphoglycerate to 3-phosphoglycerate, during the Embden-Meyerhof-Parnas pathway of glycolysis, in the later stages of spermatogenesis.
• Synonyms: dJ417L20.2; PGK-2; PGKB; PGKPS