PEA15 Rabbit Polyclonal Antibody

CAT#: TA379790

Phospho-PEA15-S104 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using Phospho-PEA15-S104 Rabbit pAb (TA379790) at 1:1000 dilution.C6 cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Enhanced Kit .|Exposure time: 90s.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IHC,1:50 - 1:100
• Reactivities: Human, Mouse, Rat
• Modifications: Phospho S104
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic phosphorylated peptide around S104 of human PEA15 (NP_003759.1).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 15kDa/17kDa
• Gene Name: phosphoprotein enriched in astrocytes 15
• Database Link:
  Entrez Gene 8682 Human
  UniProt ID Q15121
• Background: This gene encodes a death effector domain-containing protein that functions as a negative regulator of apoptosis. The encoded protein is an endogenous substrate for protein kinase C. This protein is also overexpressed in type 2 diabetes mellitus, where it may contribute to insulin resistance in glucose uptake. Alternative splicing results in multiple transcript variants.
• Synonyms: HMAT1; HUMMAT1H; MAT1; MAT1H; PEA-15; PED