Pericentrin (PCNT) Rabbit Polyclonal Antibody

CAT#: TA379701

PCNT Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using PCNT antibody (TA379701) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 180s.

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  Immunofluorescence analysis of U-2 OS cells using PCNT Rabbit pAb (TA379701) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Specifications

Product Data

• Applications: ICC/IF, WB
• Recommended Dilution: WB,1:1000 - 1:2000
  IF,1:50 - 1:200
• Reactivities: Human, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant protein of human PCNT.
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 378kDa
• Gene Name: pericentrin
• Database Link:
  Entrez Gene 5116 Human
  UniProt ID O95613
• Background: The protein encoded by this gene binds to calmodulin and is expressed in the centrosome. It is an integral component of the pericentriolar material (PCM). The protein contains a series of coiled-coil domains and a highly conserved PCM targeting motif called the PACT domain near its C-terminus. The protein interacts with the microtubule nucleation component gamma-tubulin and is likely important to normal functioning of the centrosomes, cytoskeleton, and cell-cycle progression. Mutations in this gene cause Seckel syndrome-4 and microcephalic osteodysplastic primordial dwarfism type II. Two transcript variants encoding different isoforms have been found for this gene.
• Synonyms: KEN; kendrin; KIAA0402; MOPD2; PCN; PCNT1; PCNT2; PCNTB; PCTN2; pericentrin; pericentrin-2; pericentrin-380; Pericentrin-B; SCKL4