IKB alpha (NFKBIA) Rabbit Polyclonal Antibody

CAT#: TA379098

IκBα Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using IκBα antibody (TA379098) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 5s.

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  Immunofluorescence analysis of NIH/3T3 cells using IκBα Rabbit pAb (TA379098) at dilution of 1:250 (40x lens). Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of PC-12 cells using IκBα Rabbit pAb (TA379098) at dilution of 1:250 (40x lens). Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of U2OS cells using IκBα Rabbit pAb (TA379098) at dilution of 1:250 (40x lens). Blue: DAPI for nuclear staining.

Specifications

Product Data

• Applications: ICC/IF, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IF,1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic peptide of human IκBα.
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 35kDa
• Gene Name: NFKB inhibitor alpha
• Database Link:
  Entrez Gene 4792 Human
  UniProt ID P25963
• Background: This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease.
• Synonyms: I-kappa-B-alpha; IkappaBalpha; IkB-alpha; IKBA; MAD-3; MAD3; NFKBI