NFIB Rabbit Polyclonal Antibody

CAT#: TA379084

NFIB Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of HeLa cells, using NFIB antibody (TA379084) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 120s.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB,1:200 - 1:2000
  IHC,1:50 - 1:200
• Reactivities: Human, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of human NFIB (NP_005587.2).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 22kDa/33kDa/47kDa/53kDa/55kDa
• Gene Name: nuclear factor I B
• Database Link:
  Entrez Gene 4781 Human
  UniProt ID O00712
• Background: Transcriptional activator of GFAP, essential for proper brain development. Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.
• Synonyms: CTF; HMGIC/NFIB; NF-I/B; NF1-B; NFI-B; NFI-RED; NFIB2; NFIB3