CD56 (NCAM1) Rabbit Polyclonal Antibody

CAT#: TA378947

NCAM1 / CD56 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using NCAM1/CD56 antibody (TA378947) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 10s.

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  Western blot analysis of extracts of SH-SY5Y cells, using NCAM1/CD56 antibody (TA378947) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 90s.

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  Immunofluorescence analysis of C6 cells using NCAM1 / CD56 antibody (TA378947) at dilution of 1:100. Blue: DAPI for nuclear staining.

Specifications

Product Data

• Applications: ICC/IF, IHC, IP, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IHC,1:100 - 1:200
  IF,1:50 - 1:200
  IP,1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 759-858 of human NCAM1 / CD56 (NP_851996.2).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 40kDa/73kDa/80kDa/83kDa/93kDa/94kDa
• Gene Name: neural cell adhesion molecule 1
• Database Link:
  Entrez Gene 4684 Human
  UniProt ID P13591
• Background: This gene encodes a cell adhesion protein which is a member of the immunoglobulin superfamily. The encoded protein is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. The encoded protein has been shown to be involved in development of the nervous system, and for cells involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance. Alternative splicing results in multiple transcript variants.
• Synonyms: CD56; MSK39; N-CAM-1; NCAM; NCAM-1