MDM2 Rabbit Polyclonal Antibody

CAT#: TA378420

Phospho-MDM2-S166 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of 293 cells, using Phospho-MDM2-S166 antibody (TA378420). 293 cells were treated by Hydroxyurea (4mM) for 20 hours.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB,1:500 - 1:1000
• Reactivities: Human, Mouse, Rat
• Modifications: Phospho S166
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic phosphorylated peptide around S166 of human MDM2 (NP_002383.2).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 11-14kDa/24-55kDa
• Gene Name: MDM2 proto-oncogene
• Database Link:
  Entrez Gene 4193 Human
  UniProt ID Q00987
• Background: This gene encodes a nuclear-localized E3 ubiquitin ligase. The encoded protein can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. This gene is itself transcriptionally-regulated by p53. Overexpression or amplification of this locus is detected in a variety of different cancers. There is a pseudogene for this gene on chromosome 2. Alternative splicing results in a multitude of transcript variants, many of which may be expressed only in tumor cells.
• Synonyms: Hdm2; HDMX; MGC5370; MGC71221; OTTHUMP00000183495