MAD2L2 Rabbit Polyclonal Antibody

CAT#: TA378192

MAD2B/MAD2L2 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using MAD2B/MAD2B/MAD2L2 antibody (TA378192) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 5s.

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  Immunofluorescence analysis of H9C2 cells using MAD2B/MAD2B/MAD2L2 antibody (TA378192) at dilution of 1:100. Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of L929 cells using MAD2B/MAD2B/MAD2L2 antibody (TA378192) at dilution of 1:100. Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of U2OS cells using MAD2B/MAD2B/MAD2L2 antibody (TA378192) at dilution of 1:100. Blue: DAPI for nuclear staining.

Specifications

Product Data

• Applications: ICC/IF, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IF,1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-211 of human MAD2B/MAD2B/MAD2L2 (NP_006332.3).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 24kDa
• Gene Name: MAD2 mitotic arrest deficient-like 2 (yeast)
• Database Link:
  Entrez Gene 10459 Human
  UniProt ID Q9UI95
• Background: The protein encoded by this gene is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. The encoded protein, which is similar to MAD2L1, is capable of interacting with ADAM9, ADAM15, REV1, and REV3 proteins.
• Synonyms: hREV7; MAD2B; OTTHUMP00000002273; REV7