Lamin B1 (LMNB1) Rabbit Polyclonal Antibody

CAT#: TA378085

Lamin B1 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using Lamin B1 Rabbit pAb (TA378085) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 1min.

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  Immunofluorescence analysis of C6 cells using Lamin B1 antibody (TA378085) at dilution of 1:100. Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of NIH/3T3 cells using Lamin B1 antibody (TA378085) at dilution of 1:100. Blue: DAPI for nuclear staining.

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  Confocal immunofluorescence analysis of HeLa cells using Lamin B1 antibody (TA378085) at dilution of 1:200. Blue: DAPI for nuclear staining.

Specifications

Product Data

• Applications: ICC/IF, IHC, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IHC,1:50 - 1:200
  IF,1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant protein of human Lamin B1 .
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 66kDa
• Gene Name: lamin B1
• Database Link:
  Entrez Gene 4001 Human
  UniProt ID P20700
• Background: This gene encodes one of the two B-type lamin proteins and is a component of the nuclear lamina. A duplication of this gene is associated with autosomal dominant adult-onset leukodystrophy (ADLD). Alternative splicing results in multiple transcript variants.
• Synonyms: ADLD; LMN; LMN2; LMNB; MGC111419