CD117 Rabbit Polyclonal Antibody

CAT#: TA377824

CD117/c-Kit Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using CD117/c-Kit antibody (TA377824) at 1:500 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic CD117/c-Kit .|Exposure time: 90s.

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  Immunofluorescence analysis of NIH/3T3 cells using CD117/c-Kit antibody (TA377824) at dilution of 1:50. Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of PC-12 cells using CD117/c-Kit antibody (TA377824) at dilution of 1:50. Blue: DAPI for nuclear staining.

Specifications

Product Data

• Applications: ICC/IF, IHC, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IHC,1:50 - 1:200
  IF,1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 25-295 of human CD117/c-Kit (NP_000213.1).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 46kDa/109kDa
• Gene Name: KIT proto-oncogene receptor tyrosine kinase
• Database Link:
  Entrez Gene 3815 Human
  UniProt ID P10721
• Background: This gene encodes the human homolog of the proto-oncogene c-kit. C-kit was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. This protein is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism. Multiple transcript variants encoding different isoforms have been found for this gene.
• Synonyms: C-Kit; CD117; PBT; SCFR