KDM1A Rabbit Polyclonal Antibody

CAT#: TA377750

KDM1 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts from normal (control) and KDM1 Rabbit pAb knockout (KO) 293T cells, using KDM1 Rabbit pAb antibody (TA377750) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 180s.

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  Western blot analysis of extracts of various cell lines, using KDM1 antibody (TA377750) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 90s.

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  Confocal immunofluorescence analysis of Hela cells using KDM1 Polyclonal Antibody (TA377750) at dilution of 1:200. Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of HeLa cells using KDM1 Polyclonal Antibody (TA377750) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of L929 cells using KDM1 Polyclonal Antibody (TA377750) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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Specifications

Product Data

• Applications: ICC/IF, IHC, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IHC,1:50 - 1:100
  IF,1:50 - 1:100
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 130-380 of human KDM1 (NP_055828.2).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 92kDa/95kDa
• Gene Name: lysine demethylase 1A
• Database Link:
  Entrez Gene 23028 Human
  UniProt ID O60341
• Background: This gene encodes a nuclear protein containing a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. Alternative splicing results in multiple transcript variants.
• Synonyms: AOF2; BHC110; KDM1; LSD1