IRF3 Rabbit Polyclonal Antibody

CAT#: TA377568

Phospho-IRF3-S396 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of NIH/3T3 cells, using Phospho-IRF3-S396 antibody (TA377568) at 1:1000 dilution.NIH/3T3 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit .|Exposure time: 180s.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB,1:500 - 1:2000
• Reactivities: Human, Mouse, Rat
• Modifications: Phospho S396
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A phospho specific peptide corresponding to residues surrounding S396 of human IRF3
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 12kDa/16kDa/33kDa/47kDa/49kDa
• Gene Name: interferon regulatory factor 3
• Database Link:
  Entrez Gene 3661 Human
  UniProt ID Q14653
• Background: This gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
• Synonyms: IRF-3