IFITM3 Rabbit Polyclonal Antibody

CAT#: TA377345

IFITM3 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts from normal (control) and IFITM3 knockout (KO) HeLa cells, using IFITM3 antibody (TA377345) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 1s.

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  Western blot analysis of extracts of HeLa cells, using IFITM3 antibody (TA377345) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 90s.

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  Immunofluorescence analysis of C6 cells using [KO] IFITM3 antibody (TA377345) at dilution of 1:100. Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of L929 cells using [KO] IFITM3 antibody (TA377345) at dilution of 1:100. Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of U-2 OS cells using [KO] IFITM3 antibody (TA377345) at dilution of 1:100. Blue: DAPI for nuclear staining.

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Specifications

Product Data

• Applications: ICC/IF, IHC, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IHC,1:50 - 1:200
  IF,1:50 - 1:100
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-133 of human IFITM3 (NP_066362.2).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 14kDa
• Gene Name: interferon induced transmembrane protein 3
• Database Link:
  Entrez Gene 10410 Human
  UniProt ID Q01628
• Background: The protein encoded by this gene is an interferon-induced membrane protein that helps confer immunity to influenza A H1N1 virus, West Nile virus, and dengue virus. Two transcript variants, only one of them protein-coding, have been found for this gene. Another variant encoding an N-terminally truncated isoform has been reported, but the full-length nature of this variant has not been determined.
• Synonyms: 1-8U; interferon-inducible; IP15