HMG1 (HMGB1) Rabbit Polyclonal Antibody

CAT#: TA377132

Acetyl-HMGB1-K29 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts from normal (control) and Acetyl-HMGB1-K29 knockout (KO) 293T cells, using Acetyl-HMGB1-K29 antibody (TA377132) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit .|Exposure time: 5min.

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  Immunofluorescence analysis of C6 cells using [KO Validated] Acetyl-HMGB1-K29 Rabbit pAb (TA377132) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of NIH-3T3 cells using [KO Validated] Acetyl-HMGB1-K29 Rabbit pAb (TA377132) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of U-2 OS cells using [KO Validated] Acetyl-HMGB1-K29 Rabbit pAb (TA377132) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Specifications

Product Data

• Applications: ICC/IF, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IF,1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Modifications: Acetyl K29
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic acetylated peptide around K29 of human HMGB1.
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 24kDa
• Gene Name: high mobility group box 1
• Database Link:
  Entrez Gene 3146 Human
  UniProt ID P09429
• Background: This gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein.
• Synonyms: Amphoterin; DKFZp686A04236; HMG-1; HMG1; HMG3; OTTHUMP00000018196; OTTHUMP00000190860; OTTHUMP00000200117; SBP-1