Histone H2A.X (H2AFX) Rabbit Polyclonal Antibody

CAT#: TA377049

Phospho-Histone H2AX-S139 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of NIH/3T3 cells, using Phospho-Histone H2AX-S139 antibody (TA377049) at 1:500 dilution.NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 180s.

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  Western blot analysis of extracts of C6 cells, using Phospho-Histone H2AX-S139 antibody (TA377049) at 1:500 dilution.C6 cells were treated by UV at room temperature for 15-30 minutes.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit .|Exposure time: 180s.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB,1:500 - 1:1000
• Reactivities: Human, Mouse, Rat
• Modifications: Phospho S139
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic phosphorylated peptide around S139 of human Histone H2AX (NP_002096.1).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 15kDa
• Gene Name: H2A histone family member X
• Database Link:
  Entrez Gene 3014 Human
  UniProt ID P16104
• Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.
• Synonyms: H2A.X; H2A/X; H2AX