FOXO3 Rabbit Polyclonal Antibody

CAT#: TA376340

Phospho-FOXO3A-S253 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of 293T cells, using Phospho-FOXO3A-S253 antibody (TA376340) at 1:2000 dilution.293T cells were treated by 10% FBS at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 10s.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB,1:500 - 1:2000
• Reactivities: Human, Mouse, Rat
• Modifications: Phospho S253
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic phosphorylated peptide around S253 of human FOXO3A (NP_001446.1).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 48kDa/71kDa
• Gene Name: forkhead box O3
• Database Link:
  Entrez Gene 2309 Human
  UniProt ID O43524
• Background: This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed.
• Synonyms: AF6q21; DKFZp781A0677; FKHRL1; FKHRL1P2; FOXO2; FOXO3A; MGC12739; MGC31925