Eph receptor A2 (EPHA2) Rabbit Polyclonal Antibody

CAT#: TA375927

Phospho-EphA2-Y594 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of SH-SY5Y cells, using Phospho-EphA2-Y594 antibody (TA375927) at 1:1000 dilution. SH-SY5Y cells were treated by EphrinA1 (100 ng/mL) for 5 minutes .|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit .|Exposure time: 60s.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB,1:500 - 1:2000
• Reactivities: Human, Rat
• Modifications: Phospho Y594
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic phosphorylated peptide around Y594 of human EphA2 (NP_004422.2).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 54kDa/108kDa
• Gene Name: EPH receptor A2
• Database Link:
  Entrez Gene 1969 Human
  UniProt ID P29317
• Background: This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. Mutations in this gene are the cause of certain genetically-related cataract disorders.
• Synonyms: ARCC2; ECK