EGR1 Rabbit Polyclonal Antibody

CAT#: TA375768

EGR1 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using EGR1 antibody (TA375768) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit .|Exposure time: 10s.

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  Immunofluorescence analysis of U2OS cells using EGR1 Rabbit pAb (TA375768) at dilution of 1:250 (40x lens). Blue: DAPI for nuclear staining.

Specifications

Product Data

• Applications: ICC/IF, WB
• Recommended Dilution: WB,1:200 - 1:1000
  IF,1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant protein of human EGR1
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 57kDa
• Gene Name: early growth response 1
• Database Link:
  Entrez Gene 1958 Human
  UniProt ID P18146
• Background: The protein encoded by this gene belongs to the EGR family of C2H2-type zinc-finger proteins. It is a nuclear protein and functions as a transcriptional regulator. The products of target genes it activates are required for differentitation and mitogenesis. Studies suggest this is a cancer suppressor gene.
• Synonyms: AT225; EGR-1; G0S30; KROX-24; NGFI-A; TIS8; ZIF-268; ZNF225