DET1 Rabbit Polyclonal Antibody

CAT#: TA375425

DET1 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using DET1 antibody (TA375425) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit .|Exposure time: 10s.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB,1:500 - 1:2000
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 15-240 of human DET1 (NP_060466.2).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 63kDa/65kDa
• Gene Name: de-etiolated homolog 1 (Arabidopsis)
• Database Link:
  Entrez Gene 55070 Human
  UniProt ID Q7L5Y6
• Background: Component of the E3 ubiquitin ligase DCX DET1-COP1 complex, which is required for ubiquitination and subsequent degradation of target proteins. The complex is involved in JUN ubiquitination and degradation.
• Synonyms: FLJ10103; MGC126156; MGC126157